| Numéro |
J. Phys. Colloques
Volume 47, Numéro C8, Décembre 1986
EXAFS and Near Edge Structure IV
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|---|---|---|
| Page(s) | C8-1173 - C8-1176 | |
| DOI | https://doi.org/10.1051/jphyscol:19868229 | |
EXAFS and Near Edge Structure IV
J. Phys. Colloques 47 (1986) C8-1173-C8-1176
DOI: 10.1051/jphyscol:19868229
1 Department of Chemistry, University of Manchester, GB-Manchester M13 9PL, Great-Britain
2 Synchrotron Radiation Source, SERC Daresbury Laboratory, GB-Warrington WA4 4AD, Great-Britain
3 Gorlaeus Laboratories, State University, P.O. Box 9502, NL-2300 RA Leiden, The Netherlands
4 Department of Biochemistry and Biophysics, Chalmers University of Technology/University of Gothenburg, S-41296 Gothenburg, Sweden
J. Phys. Colloques 47 (1986) C8-1173-C8-1176
DOI: 10.1051/jphyscol:19868229
CHANGES IN THE METAL COORDINATION SPHERE OF AZURIN AND STELLACYANIN UNDER INFLUENCE OF pH, OXIDATION/REDUCTION AND METAL SUBSTITUTION
M.C. FEITERS1, 2, S.S. HASNAIN2, C.M. GROENEVELD3, G.W. CANTERS3, J. VAN RIJN3, J. REEDIJK3, S. DAHLIN4 et B. REINHAMMAR41 Department of Chemistry, University of Manchester, GB-Manchester M13 9PL, Great-Britain
2 Synchrotron Radiation Source, SERC Daresbury Laboratory, GB-Warrington WA4 4AD, Great-Britain
3 Gorlaeus Laboratories, State University, P.O. Box 9502, NL-2300 RA Leiden, The Netherlands
4 Department of Biochemistry and Biophysics, Chalmers University of Technology/University of Gothenburg, S-41296 Gothenburg, Sweden
Résumé
La coordination de métal dans des protéines à cuivre bleu, azurine et stellacyanine, a été étudié par EXAFS. Le pH n'a pas beaucoup d'influence sur la coordination de cuivre dans azurine. Dans azurine comme dans stellacyanine, l'échelonnement des distances Cu-N (His) grandit avec réduction, et un ligant soufre outre le ligant S(Cys) est observé.
Abstract
The metal coordination sphere in the blue copper proteins, azurin and stellacyanin, has been studied by EXAFS. The pH has little influence on the copper coordination in azurin. In both azurin and stellacyanin, the spread in the Cu-N(His) bonds increases upon reduction and a sulphur ligand in addition to S(Cys) is observed.