Issue
J. Phys. Colloques
Volume 40, Number C2, Mars 1979
International Conference on The Applications of The Mössbauer Effect
Page(s) C2-495 - C2-497
DOI https://doi.org/10.1051/jphyscol:19792173
International Conference on The Applications of The Mössbauer Effect

J. Phys. Colloques 40 (1979) C2-495-C2-497

DOI: 10.1051/jphyscol:19792173

MÖSSBAUER SPECTROSCOPY OF THE MODEL HEMOGLOBIN COMPOUND DICHELATED PROTOHEME

E.V. Mielczarek1, B. Balko2, R.L. Berger1, D.K. White3, T.G. Traylor3 and T. Mincey3

1  Physics Department, George Mason University, Fairfax, Va. 22030, U.S.A. and Laboratory of Techical Development, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. 20014, U.S.A.
2  Laboratory of Technical Development, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. 20014, U.S.A.
3  Department of Chemistry, University of California at San Diego, La Jolla, Calif. 92093, U.S.A.


Résumé
Des mesures Mössbauer sur la protohème chélatée (protohème-di-3-(1-imidazole propylamide) ont été effectuées à des températures allant de l'ambiante à 5.4 K. A la température ambiante, le composé présente un doublet quadripolaire bien résolu avec dédoublement de raie de 1,954 ± 0.004 mm s-1 et un déplacement isomérique de +0,184 ± 0,014 mm s-1 par rapport au Fe normal. Ce dédoublement bien résolu persiste jusqu'à 17 K. A 17 K, les deux raies s'élargissent dans un rapport de trois à un, la raie d'énergie supérieure diminuant d'intensité par rapport à l'autre.


Abstract
Mössbauer measurements of dichelated protoheme (protoheme-di-3-(I-imidazole propylamide) have been made from room temperature to 5.4 K. The compound shows a well-resolved quadrupole doublet at room temperature with a line splitting of 1.954 ± 0.014 mm s-1 and an isomer shift of +0.184 ± 0.014 mm s-1 with respect to natural Fe. This well-resolved splitting persists down to 17 K. At 17 K the onset of a hyperfine interaction is evident with both lines broadening by a factor of three with the higher velocity line decreasing in intensity with respect to the lower velocity line.